In contrast to wild-type tumors with no thrombospondin-1 expression, a tumor-suppressive index was demonstrated only in mice bearing D-12, U-25, or thrombospondin-1 overexpressing R-18 tumors, as validated by melanoma angiogenesis, lung colonization, and spontaneous pulmonary metastasis [32]. The gene discussed is THBS1; the disease is melanoma.