Data from LDH release assay showed that cell cytotoxicity, though increasing with the prolonged viral infection time, did not reach significant difference in IECs at MOI 5 for 2–48 hpi (48 hpi: 4.8% ± 1.5%, p value = 0.09), as seen in Figure 4C. In addition, the mRNA expression of host antiviral innate immunity genes Ifnβ, Cxcl10, and Mx1 were induced by AiV infection, as seen in Figure 4D–F. Here, MX1 is linked to viral infectious disease.