Cells were treated according to the description in Figure 1A. As shown in Figure 4C, we found that RT + BEZ235 treatment followed by BEZ235 maintenance treatment showed prolonged DNA damage (more 53BP1 foci) in three CRC cell lines until day 7 when compared with the RT + BEZ235, RT, BEZ235, BEZ235 + mBEZ235, and control treatments. Here, TP53BP1 is linked to colorectal carcinoma.