In addition to VEGF-A, immunoblotting revealed that HIF-1α expression was significantly downregulated after RT + BEZ235 + mBEZ235 treatment when compared with the RT and RT + BEZ235 treatments in all CRC cells (HCT116, HT29, and SW480 cells) (Figure 3C). This evidence concerns the gene HIF1A and colorectal carcinoma.