In addition, upregulation of the expression of NKILA resulted in retention of most of the P65 in the cytoplasm upon TNF-α stimulation, In contrast, the depletion of NKILA expression significantly prolonged the sustained activation time of NF-κB pathway, our study firstly demonstrated that NKILA can regulate the NF-κB activation in NPC. This evidence concerns the gene TNF and nasopharyngeal carcinoma.