However, age showed no significant difference between CCN1 high or CCN1 low patient group in LUSC and STAD cohorts, whereas, in BLCA, age and stage of cancer showed significant (p<0.05) difference or enrichment between CCN1 high and CCN1 low patient groups, suggesting potential association of age and stage of cancer with patient survival in BLCA (Figure 7—figure supplement 1F–H). This evidence concerns the gene CCN1 and bladder transitional cell carcinoma.