We further verified the expression of both cytolytic genes (GZMA and PRF1), MMR-related genes (MLH1, MSH2, MSH6 and PMS2), immune checkpoint molecules (PD-L1/2, CTLA-4, IDO1, TIGIT, LAG3, VISTA), as well as that of CD8 and FOXP3 (Treg marker) in an independent cohort of 72 colorectal adenocarcinomas by RT-qPCR (Fig. 6a). The gene discussed is PRF1; the disease is colorectal adenocarcinoma.