Similarly, CYT-high rectal cancers correlated with mutations in PHF14, LRRFIP2, SERBP1, CD58, PTEN, CD4, SPZ1, PKNOX1 and SETD4, and CYT-low rectum tumors were associated with missense, nonsense or splice-site mutations in TCF7L2, TP53, SMAD2, PCBP1, ZC3HAV1 and KCNB2. Significantly mutated genes (FDR < 0.1) including APC, FBXW7, ST8SIA6, PPP2R2C and FGF13 were detected in equal frequencies between both cytolytic subgroups of the READ dataset (Fig. 3f). This evidence concerns the gene SETD4 and rectal neoplasm.