To test whether high-salt induces endothelial NLRP3 expression and IL-1β secretion in vitro, HUVECs were cultured in media with different osmolarities, ranging from 270 mosmol/kg (lower end of the normal physiological range) to 290, 310, 330, and 350 mosmol/kg (hypernatremia). This evidence concerns the gene NLRP3 and Hypernatremia.