NSUN5 and central nervous system cancer: We found the 5′-end region of NSUN5 of the LN229, A172, and KS-1 glioma cell lines to be hypermethylated in comparison with normal brain white matter (Fig. 1c), whereas the DBTRG-05MG, CAS-1, and MO59 J cells were unmethylated (Fig. 1c).