We next performed comparative bisulfite sequencing of multiple clones of the 28S rRNA extracted from the NSUN5 unmethylated glioblastoma cell lines DBTRG-05MG, MO59J, and CAS-1, and the NSUN5 hypermethylated and transcriptionally silenced LN229, A172, and KS-1 glioblastoma cell lines. Here, NSUN5 is linked to glioblastoma.