The study of subcutaneously injected cancer cells into nude mice provided similar results: tumors originated from NSUN5-transfected LN229 cells had a significantly lower volume and weight than empty-vector-transfected-derived tumors (Fig. 2d), whereas NSUN5 shRNA-mediated depletion in the unmethylated DBTRG-05MG cell line increased the volume and weight of the derived tumors (Fig. 2e). Here, NSUN5 is linked to cancer.