First, we demonstrated by western-blot analyses that the three studied glioma cell lines with NSUN5 hypermethylation (LN229, A172, and KS-1) showed high levels of NQO1, whereas minimal expression of NQO1 was observed in the three NSUN5 unmethylated cell lines (DBTRG-05MG, MO59 J, and CAS-1) (Fig. 6c). This evidence concerns the gene NSUN5 and glioma.