The link between NSUN5 epigenetic silencing and C3782 28S rRNA hypomethylation was also observed in the grade III glioma cell lines: BT142 mut/- cells, harboring DNA methylation-associated silencing of NSUN5, showed an unmethylated C3782 28S site, whereas SW1088 and MOG-G-CCM (both unmethylated and expressing NSUN5) showed a methylated C3782 28S rRNA position (Suppl. Here, NSUN5 is linked to central nervous system cancer.