NSUN5 and glioblastoma: Using H2O2 in the medium to produce oxidative stress, we found that the three glioblastoma cell lines harboring NSUN5 epigenetic loss (LN229, A172, and KS1) show overall lower global protein synthesis than the three NSUN5 unmethylated cell lines (DBTRG-05MG, MO59 J and CAS-1) (Fig. 5a).