Therefore, to investigate whether compression-induced lactate production in CAF cells contributes to cancer progression, the proliferation of breast cancer cells was analyzed after being treated with the conditioned medium (CM) from control CAF cells, compressed CAF cells, or compressed CAF cells treated with c-Jun inhibitor or transfected with shRNA against ENO2, HK2, or PFKFB3 genes. Here, PFKFB3 is linked to breast carcinoma.