Not only is Knockdown of Sulf1, Sulf2a, and Sulf2b causative of eGFP-DBP fusion protein loss of Tg(l-fabp:eGFP-DBP) larvae indicative of proteinuria, but also it may function as a second hit to the GFB in the PAN nephrosis model. This evidence concerns the gene SULF1 and polyarteritis nodosa.