Rubicon depletion resulted in significantly reduced levels of GFP-Lc3 recruitment in response to the ΔphoP, ΔpurA, ΔsipB ΔssrB, and ΔflhD strains (Figure 3), in agreement with previous evidence for the role of LAP during infection with wild type S. Typhimurium (Masud et al., 2019). Here, MAP1LC3A is linked to infection.