To understand how deletion of netrin-1 altered the phenotype of ATMs, we performed differential gene expression analysis of the major monocyte and macrophage clusters, including monocytes (cluster 4), resident macrophages (cluster 5), the major macrophage cluster in obesity (cluster 0), and activated macrophages (cluster 9)(Figure 5). This evidence concerns the gene NTN1 and obesity due to melanocortin 4 receptor deficiency.