To understand how netrin-1 alters the phenotype of macrophages in the VAT during obesity, we performed single cell RNA-sequencing (scRNA-seq) of CD45+ cells isolated from VAT of WT and Ntn1Δmac mice fed HFD, using the 10× Genomics platform. Here, NTN1 is linked to obesity due to melanocortin 4 receptor deficiency.