Single intraperitoneal administration of NDEA significantly (p < 0.05) elevated liver function enzymes ALT, AST, GGT, LDH, ALP, TBA, TBil, and levels of tumor markers CEA, AFP, TSGF, TNF‐α in serum compared to normal control rats (Figure 2b,c), suggesting the presence of metabolic disorders in NDEA group animals. This evidence concerns the gene TNF and metabolic disease.