Single intraperitoneal administration of NDEA significantly (p < 0.05) elevated liver function enzymes ALT, AST, GGT, LDH, ALP, TBA, TBil, and levels of tumor markers CEA, AFP, TSGF, TNF‐α in serum compared to normal control rats (Figure 2b,c), suggesting the presence of metabolic disorders in NDEA group animals. Here, AFP is linked to metabolic disease.