To do this we used freshly isolated monocytes from CF patients or HC volunteers and differentiate them into either a M1 (CD14+, CD274+, CD86+, HLA-DR+ and Hi TNF) or M2 (CD14+, CD206+, HLA-DR- and Hi IL-10) phenotype as described in Figure 3A. Here, CD86 is linked to cystic fibrosis.