To determine the expression of Wee1 in human ESCC cells, we first detected the mRNA and protein expression of Wee1 in two ESCC cell lines (KYSE150 and EC109) and one immortalized human esophageal epithelial cell line (Het-1A) using qRT-PCR and immunoblotting analysis, respectively. Here, WEE1 is linked to esophageal squamous cell carcinoma.