As shown in Figures 2A–C, NSPD-12m had potent inhibitory effects on infection by all tested HIV-1 Env pseudotyped viruses, including HIV-1SF162 (R5 strain), HIV-1JR-FL (R5 strain), and HIV-1HXB2 (X4 strain), with EC50 values of approximately 19.65, 13.95, and 23.09 μM, respectively. The gene discussed is ERVW-1; the disease is infection.