KRAS does not have hotspots comparable to E132 and P185 of NRAS. The G60 hotspot, identified in other cancer types30, was not found in our study; however, mutations were enriched overall within the five amino acids immediately upstream of Q61 (amino acids 56–60, seven mutations, p = 5.1E − 04) (Fig. 2b). This evidence concerns the gene NRAS and cancer.