KRAS and colorectal carcinoma: This identified 16 mutational hotspots for KRAS (Fig. 2a); these included four sites previously reported from a dataset of >1267 CRC tumors29, which were a subset of 10 hotspots identified from an analysis of ~25,000 tumors of all types30, as well as 6 additional sites not previously apparent in analysis of smaller cohorts of CRC tumors (residues V9, V14, R68, R164, K176, and K180).