While mice fed a 60% HFD for 17 days and mice or rats fed a 40% HFD for 6–8 weeks exhibited memory impairment accompanied by increased phosphorylation at hSer616/mSer612 or hSer312/mSer307 [21,22], 12-week-old DIO mice fed a 60% HFD for 9 weeks exhibited normal memory function under our experimental conditions (Figure S1), suggesting that changes in neural IRS1 Ser residues are variable on a temporal scale in DIO mice. The gene discussed is IRS1; the disease is memory impairment.