It has been reported that GEM resistance is common in primary or acquired pancreatic cancers.9, 37 Considering that GEM is a basic therapy, several studies have explored the underlying mechanism of GEM resistance.9, 37 Our results suggested that HK2 knockdown increased cell apoptosis compared with only GEM treatment, which made pancreatic cancer cells more sensitive to GEM. This evidence concerns the gene HK2 and familial pancreatic carcinoma.