The C57BL/6 strain has been most extensively studied as a model of early-onset hearing loss, Our previously research was based on C57 early-onset hearing loss model and found expression of Cav1.3 was changes during the process of aging, Cav1.3 is mainly expressed in CNS, it has been reported that Ca2+ dysfunction plays an important role in age-related neurodegeneration [31]. Here, CACNA1D is linked to hearing loss disorder.