To explore the functional importance of NRP1 in CRC progression, we cloned NRP1 cDNA from an HCT116 CRC cell library by RT-PCR using primers at the 5′ and 3′ends of the full-length human WT NRP1 open reading frame (2772 bp cDNA encoding 923 amino acids). This evidence concerns the gene NRP1 and colorectal carcinoma.