To the best of our knowledge, we conducted the first study to determine 99th percentile URLs of hs‐cTnT under both 2018 AACC/IFCC criteria and improved selection criteria through questionnaire survey, physical examination, and laboratory screening to further exclude hypertension, overweight and obesity, and dyslipidemia, which was proven in our study that the latter criteria avoided overestimation of the former. The gene discussed is TNNT2; the disease is obesity due to melanocortin 4 receptor deficiency.