To validate the results of bioinformatic analysis, we conducted a qRT-PCR for evaluation of hsa-miR-499a-5p, hsa-miR-499a-3p, AEBP2, and ZNEF1 expression levels of tumor samples in low-tobacco, medium-tobacco, and high-tobacco exposed HNSCC patients in our hospital. The gene discussed is AEBP2; the disease is neoplasm.