To validate the results of bioinformatic analysis, we conducted a qRT-PCR for evaluation of hsa-miR-499a-5p, hsa-miR-499a-3p, AEBP2, and ZNEF1 expression levels of tumor samples in low-tobacco, medium-tobacco, and high-tobacco exposed HNSCC patients in our hospital. This evidence concerns the gene AEBP2 and head and neck squamous cell carcinoma.