In this study, we found that the invasion rate and migration rate (after 24 h) of lung cancer A549 cells following Drp1 knockdown decreased by 25% and 10%, respectively, when compared with the control group, whereas overexpression of Drp1 significantly increased the cell migration rate, indicating that Drp1 is associated with the invasion, migration, and metastasis of lung cancer cells. This evidence concerns the gene DNM1L and lung carcinoma.