CDH2 and neoplasm: Using immunofluorescence detection of EMT-related protein markers, vimentin and N-cadherin expression were significantly increased in 3D culture compared to 2D-cultivated cells, and expression of E-cadherin was decreased (Fig. 1H); thus, the 3D-culture model may facilitate cell-matrix interactions in the tumor micro- environment and promote EMT transformation of tumor cells.