After co-culture, the tumor migratory microenvironment formed by inclusion of HLF-1 cells significantly promoted the migration of A549 cells when NRP1 was highly expressed, but when NRP1 was inhibited, the number of A549 cells that migrated was close to the number of migrating cells in the HLF-1 group cultured alone. The gene discussed is NRP1; the disease is neoplasm.