Among the differentially expressed proteins identified using iTRAQ-based quantitative proteomic analysis, the expression profile of peroxiredoxin2 (PRDX2), biglycan (BGN), lumican (LUM), and serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (PPP3CA) in primary CCA tissue were examined using immunohistochemistry (IHC) assay. The gene discussed is PRDX2; the disease is cholangiocarcinoma.