Furthermore, in the fresh tumor tissues, the level of TGF-β1 in the high Lgr5 expression group were much higher compared with the low Lgr5 expression group (P < 0.0001; 194.77 ± 93.74 pg/ml vs. 105.13 ± 71.90 pg/ml; Figure 4I). This evidence concerns the gene LGR5 and neoplasm.