Here, we also demonstrated the potential of GTN to induce the breast cancer-derived cell line MDA-MB-231 to undergo necroptosis via the RIP3 pathway with increased RIP3 protein expression, whereas the rip1, rip3, and mlkl gene expressions were also significantly enhanced, finally leading to necroptosis as illustrated in Figure 6. Here, RIPK1 is linked to breast carcinoma.