The infection of 1CT and 1CTA cells with MC1 TT or MC1 ΔcdtB bacteria at MOI 25:1 resulted in the formation of Salmonella‐containing vacuoles, detected by LPS staining 24‐hr postinfection (Figure S7C), without major alteration of the epithelial layer and was therefore used in all the subsequent experiments. Here, ATP7A is linked to infection.