Importantly, its content dropped 2-8 times in cancer cells, which have gain of function mutation in the DNA binding domain of p53 (homozygous R273H mutation in HT-29 colon cancer cells or heterozygous R248Q mutation in PC-9 non-small lung cancer cells) (Figure 3A and Tables S3-S5). This evidence concerns the gene TP53 and malignant colon neoplasm.