The loss of E-cadherin expression was mostly caused by Snail expression in our study, since the Twist and Snail are the most commonly expressed TFs in HNSCC 31, and the expression levels of Twist1 were unchanged in HNSCC cells after treatment with VP when E-cadherin was upregulated (Figure S3). The gene discussed is TWIST1; the disease is head and neck squamous cell carcinoma.