In addition, we found that myocardial FGF21 production and release as well as activation of AMPK-FGF21 axis by heart are robustly induced upon myocardial ischemia in mice, and that activation of AMPK is required for catecholamine- and SFA-induced FGF21 expression in cardiac myocytes. This evidence concerns the gene FGF21 and myocardial ischemia.