The DEGs were determined to be involved in key pathways, such as oxidative phosphorylation, Parkinson’s disease, non-alcoholic fatty liver disease (NAFLD), Huntington’s disease, cardiac muscle contraction, Alzheimer’s disease, fatty acid biosynthesis, AMPK signaling pathway, insulin signaling pathway, and tight junction. The gene discussed is INS; the disease is metabolic dysfunction-associated steatotic liver disease.