Interestingly, a dramatic increase in GDF15 mRNA expression was observed following RSU-1 silencing in A172 glioma cells, which have low endogenous GDF15 levels (Figure 4D) versus non-specific control (NSC) sample, while a smaller increase was observed in H4 cells, which have higher endogenous GDF15 levels. Here, GDF15 is linked to central nervous system cancer.