Similarly, the expression of EGFR in the cell-derived EVs was significantly higher in the glioma cells U87MG (unpaired Student's t-test, **P < 0.01) and U251 (unpaired Student's t-test, **P < 0.01) than in the normal HA cells (Figure 2C, 2D), in the same tendency as the expression of EGFR in the parental cell lines, indicating that the expression of EGFR in the EVs was associated with the expression in the parental cells. This evidence concerns the gene EGFR and central nervous system cancer.