NLGN3 and central nervous system cancer: We investigated the mRNA levels of NLGN3 and PTTG1, two genes related to the proliferation and poor prognosis of glioma, in serum EVs, and found higher mRNA levels of NLGN3 and PTTG1 in serum EVs from glioma patients compared to those from normal individuals, although the expression level varies between glioma patients.