EGFR and central nervous system cancer: We found that EGFR+ EVs exhibited high sensitivity (86.96%) and specificity (83.7%) in identifying glioma patients with an area under the curve (AUC) of 0.900 (95% CI of 0.8064 ~ 0.9936, P <0.0001, Figure 3F, Table S6 in the supporting information), indicating the high diagnostic value of EGFR+ EVs in detecting glioma.