We found negligible mRNA levels of PTTG1 in EVs from healthy donors (n =9) and increased PTTG1 mRNA in EVs from glioma patients (n =18) (nonparametric test, **P <0.01), although the mRNA level of PTTG1 varied in glioma patients (Figure 5B). This evidence concerns the gene PTTG1 and glioma.