As expected, the expression of EGFR was significantly higher in the glioma cell lines U87MG (unpaired Student's t-test, ***P < 0.0001) and U251 (unpaired Student's t-test, ***P < 0.0001) than in the normal HA cell line (Figure 2A, 2B), which was in line with the previous studies showing that EGFR was highly expressed in glioma cells 48, 49. The gene discussed is EGFR; the disease is glioma.