Surprisingly, MTT, EdU and colony formation assays revealed that siPCNA or siPCNAP1 inhibited the cell proliferation, while the co-treatment of miR-154 inhibitors or overexpression of PCNA could reverse this event (Figure 6A-B, Figure S9A-C), suggesting that pseudogene PCNAP1 promotes the proliferation of hepatoma cells through miR-154/PCNA signaling in vitro. Here, PCNAP1 is linked to hepatocellular carcinoma.