HIF1A and glioblastoma: To this aim, we transfected GBM cells with the BAT-lux reporter construct, which contains 7xTCF/LEF consensus sequences 29, immunoprecipitated HIF-1α and then performed a ChIP-droplet digital PCR (Chip-ddPCR) with specific primers flanking the Wnt-responsive sequences in the plasmid (Figure S6A).