MAP1LC3A and colorectal carcinoma: To further determine whether OSI promotes autophagic flux, we used a tandem mRFP-GFP tagged LC3 construct and found that OSI-treated CRC cells displayed increased formation of red fluorescent autolysosomes (GFP−RFP+ signal), while combinatorial treatment of CQ (chloroquine) with OSI resulted in accumulation of yellow fluorescent autophagosomes (GFP+RFP+ signal) (Fig. 3a, b).