Furthermore, inhibition of class III PI3K by 3-MA23, or siRNA-mediated ATG5 or BECN1 silencing, prominently inhibited the conversion of LC3-II and the formation of LC3 puncta in OSI-treated CRC cells (Fig. 2i–m, Supplementary Fig. 3E, F). This evidence concerns the gene BECN1 and colorectal carcinoma.