Based on WB analysis and densitometric evaluation of protein bands using ImageJ software, the RasGRP4-targeting shRNA-2 was considered to be the most efficient shRNA for decreasing RasGRP4 protein expression levels in DLBCL cells (Fig. 2a, b) as well as the mRNA levels (Fig. 2c). This evidence concerns the gene RASGRP4 and diffuse large B-cell lymphoma.