To corroborate the hypothesis that such changes in 3xTg-AD mice spleen could be related with impairment of some proinflammatory parameters, Western blot analysis was performed on spleen homogenates for the expression of TNFSF10 and its receptor TNFRSF10B, and that of the inflammatory molecules COX2, iNOS, IL-1β, TNF-α, the anti-inflammatory cytokine IL-10, as well as that of the Treg lymphocyte markers GITR and FoxP3. Here, TNF is linked to Alzheimer disease.