Compared to the NGS data in which all 20 tumors displayed upregulated BOP, DKC1 and RRP1B, the qPCR assays (Additional file 1: Figure S8a1–3) revealed upregulated BOP1, DKC1, and RRP1B in only 13 of 20 tumor samples, regardless of which individual reference gene or combinations of 4 reference genes were used for normalization. The gene discussed is BOP1; the disease is neoplasm.