To investigate the effect of TNS4 on the oncogenic activity of colon cancer cells, we first introduced two plasmids encoding shRNA targeting the sequences of TNS4 into LS174T, DLD1, DiFi, and WiDr cells along with a control shRNA targeting GFP by lentiviral infection and confirmed the specific ablation of TNS4 in the resulting cell lines by immunoblotting analysis. Here, TNS4 is linked to infection.