Next, to explore whether cetuximab-induced downregulation of TNS4 also occurs in the other CRC cell lines, we repeated the same experiments with three available CRC cell lines harboring different genomic alterations, which includes DLD1 (KRAS G13D), DiFi (EGFR amplification) and WiDr (BRAF V600E) cells. The gene discussed is EGFR; the disease is colorectal carcinoma.