Herein, we described two sortase A-generated TCRm-ADCs (ESK-MMAE and Q2L-MMAE), against WT1 RMF/HLA-A*02:01 complex, which could specifically kill WT1 RMF/HLA-A*02:01 positive tumor cells in vitro (Figure 3C) and effectively inhibit tumor growth in the xenograft model (Figure 3D). This evidence concerns the gene HLA-A and neoplasm.