Our preliminary validation showed that AAV(1/8)-GFP-U6-mBin1-shRNA drove substantial knock-down of Bin1 expression in hippocampal organotypic brain slices, and that control AAV(1/8)-GFP-U6-scrmb-shRNA and AAV(1/8)-GFP-U6-mBin1-shRNA infection resulted in similar distribution of GFP immunoreactivity (Fig 1C). Here, BIN1 is linked to infection.