Compared with HP, SEPT9 and SDC2 methylation levels were significantly higher in CRC cancer tissues (P < .01, Figure 1C,D) but showed no significant difference in adenomatous polyps when compared with CRC cancer tissues (P > .05, Figure 1C,D), thus making ColoDefense test a candidate screening method for CRC and precancerous lesions. Here, SEPTIN9 is linked to colorectal carcinoma.