The requirement of SMARCA4 ATPase and bromodomain function in SMARCA2-deficient ESCC cells was further assessed by generating siRNA/sgRNA-resistant SMARCA4 (SMARCA4res) expression constructs harboring loss-of-function mutations within the DEXDc- (K785A) and bromodomains (N1540A)41,42 (Fig. 3A). Here, SMARCA2 is linked to esophageal squamous cell carcinoma.