Again, the number of events falling under our definition for MP, i.e. within the FSc/SSc range of the 0.9 μm beads, positive for annexin-V and therefore expressing negatively charged phospholipids, and positive for CFSE and therefore representing closed vesicles enclosing cytoplasmic molecules, are increased in both cell types by ACh stimulation (1 mM) by 212% and 414%, respectively; on average, tiotropium inhibits the effect almost completely. The gene discussed is ANXA5; the disease is systemic sclerosis.