PRKN and neuroblastoma: Treatment of primary rat cortical neurons, human SH-SY5Y neuroblastoma cells, and immortalized mouse embryonic fibroblasts (MEFs) with 200 μM L-DOPA for 24 hours led to a statistically significant loss of parkin in each case (cortical neurons: 13.6 ± 5.2% parkin loss, p = 0.048, N = 6; SH-SY5Y: 32.7 ± 3.8% parkin loss, p = 0.001, N = 5; MEFs: 31.3 ± 12.0% parkin loss, p = 0.048, N = 6) (Figs 1C, S2).