ALB and metabolic dysfunction-associated steatotic liver disease: To generate a 3D spheroid hepatocyte model of NAFLD, we incubated spheroids with increasing amounts of 2:1 oleic:palmitic acid-enriched medium (160 μM, 240 μM (data not shown) and 320 μM total FA concentration, conjugated to bovine serum albumin (BSA), as described previously26.