In this study, we investigated transcriptional silencing by the promoter hypermethylation of 18 tumor suppressor candidate genes (MGMT, sFRP2, HIC1, sFRP4, Timp3, sFRP5, TFPI2, p16, sFRP1, E-cad, SOX17, GATA4, GATA5, p14, FBN2, p15, and TCERG1L) in 10 OSCC cell lines and a small set of OSCC primary tumor samples (n = 33). Here, TFPI2 is linked to neoplasm.